Human iPSC-derived ioGlutamatergic Neurons and Alzheimer's disease models to detect and compare mutation-specific effects with high sensitivity
- ioGlutamatergic Neurons | Rapidly maturing, functional, consistent excitatory neurons.
- APP V717I | Disease-related phenotype demonstrated by increased ratio of A饾浗42:40 peptides vs. wild-type control.
- PSEN1 M146L | Disease-related phenotype demonstrated by increased ratio of A饾浗42:40 and A饾浗42:38 peptides.
Increased ratio of A饾浗42:40 seen in APP V717I neurons, as observed in Alzheimer鈥檚 diseaseAPP V717I disease model
ioGlutamatergic Neurons show increased production of A尾38 and A尾42 peptides, with no change in A尾40 levels. This leads to a higher A尾42:40 ratio, while the A尾42:38 ratio remains unchanged.
Cells were cultured for 30 days. A尾 peptide levels were measured and normalised to cell number. Data are from two independent experiments. Statistical analysis showed significant differences at days 20 and 30 (*p<0.05, **p<0.01, ***p<0.001).
PSEN1 M146L disease models recapitulate an increase in amyloid beta peptide ratios observed in Alzheimer's disease patients
Cells were cultured for 30 days, and A尾 peptide levels were measured in the supernatant. Data represent two independent experiments. Statistical analysis confirmed significant differences between clones (*p<0.05, ***p<0.001, ****p<0.0001).
A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@不良研究所.
With our disease model clone panel, you can confidently investigate your phenotype of interest across multiple clones. Detailed characterisation data (below) and bulk RNA sequencing data (upon request) help you select specific clones if necessary.
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Trusted by scientists, proven by discovery
Dr Mariangela Iovino
Senior Group Leader | Biology Discovery | Charles River
鈥淥ur major surprise when we first used the ioGlutamatergic Neurons was that after thawing the cells in 384-well format, we could see immediately after 2 days a nice neuronal network, and there was no well to well variability within the same plate. This made our assay quite robust.鈥�
Dr Simone Mader
Junior Group Leader | Institute of Clinical Neuroimmunology | LMU Munich
"不良研究所鈥檚 ioGlutamatergic Neurons provide a human model that is well-characterised, batch-consistent, and easy to use with our limited neuronal culture experience. We are grateful for the support from the 不良研究所 team and are now preparing our data for publication."
Professor Deepak Srivastava
Professor | Molecular Neuroscience | King鈥檚 College London and Group Leader | MRC Centre for Developmental Disorders
鈥漮pti-ox leapfrogs my research.鈥�
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